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Image Search Results
Journal: JCI Insight
Article Title: Thrombomodulin protects against acute vascular and multiorgan injury in sickle cell disease
doi: 10.1172/jci.insight.193884
Figure Lengend Snippet: ( A ) Fluorescence intensity of fibrinogen and C3 complement were increased in cell-free hemoglobin–challenged SCD mice; levels were reduced after rescue with TM, similar to control conditions ( n = 8; 4 male, 4 female mice per condition; Mann-Whitney U test) (original magnification, ×400). ( B ) Activated protein C concentrations were unchanged with cell-free hemoglobin challenge and increased with TM rescue and ( C ) thrombin-antithrombin complexes increased with cell-free hemoglobin challenge and were improved with TM rescue ( n = 6; 3 male, 3 female per condition; Mann-Whitney U test). Increases in vascular injury biomarkers ( D ) VCAM-1, ( E ) vWF, ( F ) VEGF, ( G ) E-selectin, and ( H ) endothelin-1 were observed in cell-free hemoglobin–challenged mice; those that received TM rescue had levels similar to control conditions ( D – F : n = 8, 4 male, 4 female mice per condition; G and H : n = 6, 3 male, 3 female per condition; Mann-Whitney U test). Mice were challenged with cell-free hemoglobin (0.24 g/kg i.v.) and rescued with TM (5 mg/kg s.c. + 1 mg/kg i.v.) under the respective conditions, and samples and tissue were harvested 24 hours after TM rescue (26 hours for the 2-hour TM rescue and 48 hours for the 24-hour TM rescue after cell-free hemoglobin challenges). Scale bars: 50 μm. Median and IQR values provided in ; * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: Plasma concentrations of hemoglobin (E88-134, Bethyl Laboratories), TM (ab209880, Abcam), neutrophil elastase (MELA20, R&D Systems), activated protein C (ABIN6953414, Antibodies-online Inc.), thrombin-antithrombin complexes (TAT, ab230933, Abcam), VCAM-1 (ab100750, Abcam), vWF (NBP2-68175, Novus Biologicals), VEGF (MMV00, R&D Systems),
Techniques: Fluorescence, Control, MANN-WHITNEY